Device

Part:BBa_K1585351:Design

Designed by: Michael Osthege, Sarah Lubjuhn, Laura Helleckes   Group: iGEM15_Aachen   (2015-09-06)


glgP targeting plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 375
    Illegal XbaI site found at 381
    Illegal SpeI site found at 132
    Illegal SpeI site found at 266
    Illegal PstI site found at 393
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 375
    Illegal NheI site found at 155
    Illegal NheI site found at 243
    Illegal SpeI site found at 132
    Illegal SpeI site found at 266
    Illegal PstI site found at 393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 375
    Illegal BglII site found at 1238
    Illegal BamHI site found at 23
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 375
    Illegal XbaI site found at 381
    Illegal SpeI site found at 132
    Illegal SpeI site found at 266
    Illegal PstI site found at 393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 375
    Illegal XbaI site found at 381
    Illegal SpeI site found at 132
    Illegal SpeI site found at 266
    Illegal PstI site found at 393
    Illegal AgeI site found at 1088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli. It has to be used together with pCas (https://www.addgene.org/62225/)


Source

The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence.

References

http://www.ncbi.nlm.nih.gov/pubmed/25636838